rabbit polyclonal anti rad51 antibody  (Proteintech)

Journal: eLife

Article Title: Synergistic effect of inhibiting CHK2 and DNA replication on cancer cell growth

doi: 10.7554/eLife.104718

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-RAD51 , PC130 , Millipore , 1/500.

Techniques:

Journal: Genes

Article Title: B Chromosome Transcriptional Inactivation in the Spermatogenesis of the Grasshopper Eyprepocnemis plorans

doi: 10.3390/genes15121512

Figure Lengend Snippet: Meiotic reciprocal recombination in B1 chromosomes. Double immunolabeling of SMC3 (white) and RAD51 (red) in squashed spermatocytes counterstained with DAPI (blue) in individuals without B1 chromosomes (0B) and bearing one B1 (1B) or two B1 chromosomes (2B). The positions of the sex chromosome (X), the B1 univalent (B), the B1 bivalent (B II ) and autosomal bivalents (Au) are indicated. Note that in prophase I stages, the X and B1 chromosomes are heteropycnotic positive chromatin bodies after DAPI staining. ( A ) Pachytene spermatocyte of a 0B male. RAD51 foci are visible along paired SMC3 axes of autosomes, but not over the X chromosome. ( B ) Pachytene spermatocyte of a 1B male. RAD51 foci are located over the fully paired SMC3 axes of autosomes but not over the unpaired SMC3 axes of the X and B1 chromosomes. ( C ) 300% magnification of the nuclear region occupied by the X and B1 chromosomes (yellow arrowheads signal RAD51 focus on an autosomal bivalent). ( D ) Zygotene spermatocyte of a 2B male. RAD51 foci were found over paired and unpaired SMC3 axes of autosomal bivalents and the B1 bivalent. ( E ) 300% magnification of the nuclear region occupied by the X chromosome and the B1 bivalent. Yellow and green arrowheads signal RAD51 foci located on autosomal and B1 bivalents, respectively. ( F ) Early/mid pachytene spermatocyte of a 2B male. Autosomal and B1 bivalents displayed RAD51 foci over their paired SMC3 axes. ( G ) 300% magnification of the nuclear region occupied by the X chromosome and the B1 bivalent. Yellow and green arrowheads signal RAD51 foci located on autosome and B bivalents, respectively. ( H ) Metaphase I spermatocyte of a 2B1 male. SMC3 appeared at the interchromatid domain of autosomal and B1 bivalents and was interrupted at chiasma sites. ( I ) 300% magnification of the X chromosome and the B1 bivalent. Bar in ( A ), 10 µm.

Article Snippet: A rabbit polyclonal anti-RAD51 antibody (Oncogene Research Products, La Jolla, CA, USA, Ab-1, PC130), generated against recombinant HsRad51 protein, was used at 1:50 dilution.

Techniques: Immunolabeling, Staining